Revealing diets of wild-caught ornate spiny lobster, Panulirus ornatus, at puerulus, post-puerulus and juvenile stages using environmental DNA (eDNA) metabarcoding

Diets are a critical factor in the artificial production of lobster larvae, yet knowledge diet requirements spiny especially at its early life stages is rarely investigated. Thus, present study aimed finding potential diets larva by analyzing stomach content wild-caught ornate lobster, Panulirus ornatus, three different stages: puerulus, post-puerulus, and juvenile using eDNA metabarcoding. The results showed that 10 plankton species were identified puerulus stage, top five Oithona sp. (36.30% relative quantity eDNA), Macropthalmus setous (19.18%), Audacallichirus mirim (13.01%), simplex (5.48%), Pseudodiaptomus euryhalinus (4.11%). Furthermore, 17 from post-puerulus most dominant (28.60%), (19.36%), Pichia (5.96%), Helice tientsinensis (5.86%), (5.36%). At 34 identified, which (80.88%), followed Canthocalanus pauper (5.66%), Acartia bispinosa (4.02%), Longipedia koreana (2.30%), davisae (1.92%). In addition, 56 natural habitat including Sicyonia laevigata (33.73%), (23.70%), (17.70%), tonsa (11.89%). Of species, considered highly for developing artificially producing seeds sp., simplex, bispinosa, euryhalinus.